PIRCHE launches new version of TxPredictor with several enhanced capabilities

We are pleased to announce the release of a new version of PIRCHE’s TxPredictor software. This update incorporates several features that make it easier for users to define serological antigens, provides improved performance of the B-cell matching algorithm (Snow), expands coverage of the binding prediction module (Frost) to include 13 loci, and offers enhanced display capabilities within the platform’s RAMP function.  Continue reading to learn more about how they can aid workflow efficiencies and support better clinical decision-making.

HLA Input Parser v2

>     Users can now explicitly define serological antigens across all entry points of the application (e.g. DR11, DQ7), including the API, batch modules, and input wizard.

>     The original parser (v1) remains supported to ensure a smooth, backwards-compatible transition.

Improved B cell Calculations (Snow v1.1)

>     PIRCHE B-cell epitope matching now addresses homozygous alleles (or those with identical amino acid configurations) more accurately via single count, resolving potential overestimation.

>     This update has resulted in refined thresholds for surface accessibility and protrusion:

o  Surface accessibility: 0.26

o  Protrusion rank: 0.68

>     Full technical details are available via the following link: (https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2025.1548934/full).

Expanded Locus Coverage(Frost Binding Predictor)

>     The PIRCHE TxPredictor platform can now display predicted peptides that originate from 13 loci: A, B, C, DRB1, DRB3, DRB4, DRB5, DQA1, DQB1, DPA1, DPB1, MICA, MICB.

o  Currently, only DRB1 and DRB345 loci are used as presenting alleles.

o  Support for DQ and DP dimer presentation is in progress.

>     Condensed DRB1 presentation heat maps on the SOT Single Patient results screen and PDF reports now display 11 loci.

Improved Data Visualization (RAMP)

>     Acceptable mismatch bar charts now display alleles based on single antigen specificities from common single antigen bead assays (previously based on common alleles in the virtual population).

>     Locus-specific ranks are now shown in both PIRCHE-II and Snow heat maps.

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To explore these features in more detail, log into your PIRCHE account or reach out to your PIRCHE representative directly.

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Warm regards,

Team PIRCHE

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